Key result
The 1,300 base pairs of the 5'-flanking region of the human alpha-skeletal actin gene directed high-level transient expression in differentiated myotubes but not in nonmuscle cells.
Population
Myogenic (mouse C2C12 and rat L8 myotubes) and nonmyogenic (mouse L.TK- and human HuT-12) cell lines
Comparison
Transfection with human alpha-skeletal actin… vs Nonmyogenic promoter-CAT constructs and…
Design
Preclinical
Authors
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Promoter fragment enables muscle-specific expression in vitro; leaves open translation to cardiac gene regulation and in vivo models.
Multiple 5'-flanking regions of the human alpha-skeletal actin gene synergistically modulate muscle-specific expression through complex interactions with trans-acting factors.
Muscat et al. (1987) studied this question. Transfection of human alpha-skeletal actin gene 5'-flanking regions vs. Nonmuscle cells or basal promoter constructs was evaluated on CAT activity (transient expression). The 1,300 base pairs of the 5'-flanking region of the human alpha-skeletal actin gene directed high-level transient expression in differentiated myotubes but not in nonmuscle cells.