What question did this study set out to answer?

The study aims to understand how upstream regions of the human cardiac actin gene regulate its transcription in muscle cells.

June 1, 1986Molecular and Cellular Biology

Upstream Regions of the Human Cardiac Actin Gene That Modulate Its Transcription in Muscle Cells: Presence of an Evolutionarily Conserved Repeated Motif

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Population

Cultured cell lines and human heart/skeletal muscle RNA.

Comparison

Transfection with plasmids containing upstream… vs Transfection with control plasmids or…

Design

Preclinical

Authors

AMA MintySanofi (France)LKLarry KedesUniversity of Southern California

Discussion

Member takes

Implication

Does not inform clinical practice; leaves open the functional role of these elements in human cardiac disease models.

Key Points

The study aims to understand how upstream regions of the human cardiac actin gene regulate its transcription in muscle cells.
Transfection of cultured cell lines, including human heart and skeletal muscle samples.
Deletion analysis of transcriptional regulatory regions to identify sequence elements involved.
Testing the regulatory impact of identified elements on transcription using a heterologous promoter.
Identified two key sequence elements that enhance transcription when tested together; one is between -443 and -395 and another between -177 and -118.
High conservation of regulatory regions in human, mouse, and chick genes was noted.
An evolutionarily conserved repeated motif was found, suggesting significance in transcription regulation of actin and other proteins.

Structured PICO

Population

Cultured cell lines (mouse C2C12 muscle cells, mouse L fibroblasts, rat PC-G2 pheochromocytoma cells) and human heart/skeletal muscle RNA.

Intervention

Transfection with plasmids containing upstream regions of the human cardiac actin gene linked to the bacterial chloramphenicol acetyltransferase (CAT) gene.

Comparator

Transfection with control plasmids (e.g., pSV2CAT, pHCA0CAT) or transfection into non-muscle cell lines.

Outcome

Transcriptional activity measured by transient expression of the bacterial chloramphenicol acetyltransferase (CAT) gene.surrogate

The human cardiac actin gene contains evolutionarily conserved upstream regulatory elements that drive tissue-specific transcription in muscle cells.

Cite This Study

Minty et al. (1986) studied this question.

synapsesocial.com/papers/6a71e3a6e5469ee92be22e17 https://doi.org/10.1128/mcb.6.6.2125-2136.1986

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Upstream Regions of the Human Cardiac Actin Gene That Modulate Its Transcription in Muscle Cells: Presence of an Evolutionarily Conserved Repeated Motif

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Upstream Regions of the Human Cardiac Actin Gene That Modulate Its Transcription in Muscle Cells: Presence of an Evolutionarily Conserved Repeated Motif

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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