Key result
Two CArG box domains at -140 and -100 bp in the human alpha-cardiac actin gene promoter act synergistically and mutually dependently as tissue-specific regulatory upstream elements for transcription.
Population
Muscle cells expressing a bacterial chloramphenicol acetyltransferase gene directed by the human…
Comparison
Promoter region modifications targeting CArG… vs Unmodified promoter or single CArG box disruption
Design
Preclinical
Authors
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Advances molecular basis of cardiac gene regulation; leaves open therapeutic translation pending human validation.
Two CArG box domains in the human alpha-cardiac actin gene promoter act synergistically as tissue-specific regulatory elements for muscle-specific expression.
Miwa et al. (1987) studied Gene expression in muscle cells. Promoter region modifications (5' deletions, linker-scanning mutations, insertion-deletion mutations) was evaluated on Transcriptional activity of the human alpha-cardiac actin gene. Two CArG box domains at -140 and -100 bp in the human alpha-cardiac actin gene promoter act synergistically and mutually dependently as tissue-specific regulatory upstream elements for transcription.
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