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THE PH of blood was measured at body temperature by the glass electrode method about 2 minutes after the withdrawal of the blood from the blood- vessel. The PH was observed to decrease by about 0 05 a few minutes later. This acid change seemed to be very important, as it meant that later deter- minations do not represent the PH of the blood in the body. A typical PHtime curve is shown in Fig. This pH change was investigated further.
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Havard et al. (1929) studied this question.
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