What question did this study set out to answer?

This research aims to understand how thyroid hormone and retinoic acid influence gene expression in cardiac myocytes.

Influence of thyroid hormone and retinoic acid on slow sarcoplasmic reticulum Ca2+ ATPase and myosin heavy chain alpha gene expression in cardiac myocytes. Delineation of cis-active DNA elements that confer responsiveness to thyroid hormone but not to retinoic acid

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Randomized trial investigates gene expression influenced by thyroid hormone in cardiac myocytes, suggesting distinct regulatory mechanisms.

This research aims to understand how thyroid hormone and retinoic acid influence gene expression in cardiac myocytes.
Used primary neonatal rat cardiocytes in both serum-containing and serum-free media.
Conducted transient transfection experiments with Ca2+ ATPase promoter segments fused to a reporter gene.
Examined the response of mRNA encoding Ca2+ ATPase and myosin heavy chain alpha to thyroid hormone and retinoic acid.
Thyroid hormone (T3) induced Ca2+ ATPase mRNA expression with an EC50 of approximately 30 pM, while retinoic acid (RA) had an EC50 of approximately 2 nM.
Distinct response elements were identified for T3, while RA did not regulate these constructs.
T3 activated gene expression through a minimal thyroid hormone response element, while RA showed a lack of effect.

Rohrer et al. (1991) studied this question.

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Randomized trial investigates gene expression influenced by thyroid hormone in cardiac myocytes, suggesting distinct regulatory mechanisms.