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March 1, 1993Genes & DevelopmentOpen Access

The role of exon sequences in splice site selection.

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Population

Mouse immunoglobulin mu (IgM) pre-mRNA model substrate for in vitro splicing

Comparison

Deletion and reinsertion of the 5' portion of… vs Wild-type or deletion constructs

Design

Preclinical

Authors

AWA WatakabeCold Spring Harbor LaboratoryKTKozo TanakaTohoku UniversityYSY. ShimuraNational Institute for Basic Biology

Discussion

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Implication

Identifies purine-rich splicing enhancer in IgM; extends exon recognition mechanisms but leaves open human disease relevance.

Structured PICO

P
Population
Mouse immunoglobulin mu (IgM) pre-mRNA model substrate for in vitro splicing
I
Intervention
Deletion and reinsertion of the 5' portion of exon M2 (purine-rich sequence)
C
Comparator
Wild-type or deletion constructs
O
Outcome
Splicing of the immediately upstream intron and assembly of the early splicing complexsurrogate

The IgM M2 exon sequence functions as a splicing enhancer (exon recognition sequence) that stimulates early splicing complex assembly, likely mediated by U1 snRNP.

Cite This Study

Watakabe et al. (1993) studied this question.

synapsesocial.com/papers/6a6f9a67c92390ac2d07a45fhttps://doi.org/10.1101/gad.7.3.407
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1The role of branchpoint and 3'-exon sequences in the control of balanced splicing of avian retrovirus RNA.1991 · 113 citations
  2. 2Control of retroviral RNA splicing through maintenance of suboptimal processing signals.1990 · 139 citations
  3. 3Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei1983 · 11,637 citations
  4. 4The length of the downstream exon and the substitution of specific sequences affect pre-mRNA splicing in vitro.1988 · 56 citations
  5. 5RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression1987 · 2,315 citations