Urine is a good source of disease biomarkers. However, urine composition can be affected by many physiological factors. In this study, urinary proteins of rats fed with milk were analyzed by LC-MS/MS to discover the effects of milk supplementation on the urine proteome. Compared with the control group, only the major urinary protein was increased in all six rats, and four proteins were found to differ in five of six rats in the milk-fed group. These results suggest that the urinary proteome tends to remain in a largely stable state when the rats are fed milk for one week instead of water based on shotgun protein identification and the label-free quantitation.
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Youhe Gao (2018) studied this question.
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