What question did this study set out to answer?

To identify the signaling pathways that regulate NAD+ metabolism and characterize the cellular mechanisms controlling nicotinamide riboside production and transport.

February 25, 2011Journal of Biological ChemistryOpen Access

Phosphate-responsive Signaling Pathway Is a Novel Component of NAD+ Metabolism in Saccharomyces cerevisiae

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To identify the signaling pathways that regulate NAD+ metabolism and characterize the cellular mechanisms controlling nicotinamide riboside production and transport.
Established a nicotinamide riboside (NmR)-specific reporter system in Saccharomyces cerevisiae to identify genetic mutants with disrupted pyridine nucleotide metabolism.
Assayed the roles of the phosphate-responsive signaling (PHO) pathway, the vacuolar phosphatase Pho8, and the transporter Fun26 in regulating intracellular and extracellular NmR pools.
Activation of the PHO pathway increases both the internal concentration and cellular release rate of NmR, with Pho8 serving as a key vacuolar phosphatase for NmR production.
The equilibrative nucleoside transporter homolog Fun26 localizes to the vacuolar membrane to set the balance between vacuolar and cytosolic NmR pools.
Cellular depletion of nicotinic acid mononucleotide (NaMN) activates the PHO pathway to drive nicotinamide mononucleotide (NMN) catabolism for NmR salvage and phosphate recovery.

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Implication

Laboratory study reveals that phosphate signaling controls nicotinamide riboside levels in yeast, suggesting a direct link between nutrient sensing and NAD+ maintenance.

Cite This Study

Lu et al. (2011) studied this question.

synapsesocial.com/papers/6a6fa8b531a3df824327fe4c https://doi.org/10.1074/jbc.m110.217885

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Implication

Laboratory study reveals that phosphate signaling controls nicotinamide riboside levels in yeast, suggesting a direct link between nutrient sensing and NAD+ maintenance.

Phosphate-responsive Signaling Pathway Is a Novel Component of NAD+ Metabolism in Saccharomyces cerevisiae

Key Points

Discussion

Implication

Cite This Study

Phosphate-responsive Signaling Pathway Is a Novel Component of NAD+ Metabolism in Saccharomyces cerevisiae

Key Points

Discussion

Implication

Cite This Study

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