What question did this study set out to answer?

To review the structural and biochemical basis of macromolecular ligand recognition and divalent cation regulation across the integrin receptor family.

July 1, 2000Journal of Biological ChemistryOpen Access

Ligand Binding to Integrins

View Full Paper

Ask AI

Bookmark

Authors

EPEdward F. PlowVascular Medicine THThomas A. HaasCleveland Clinic LZLi ZhangKanazawa University

Discussion

Member takes

Implication

Review uncovers structural mechanisms of ligand recognition by integrin heterodimers, indicating critical coordination by divalent cation binding sites.

Key Points

To review the structural and biochemical basis of macromolecular ligand recognition and divalent cation regulation across the integrin receptor family.
Synthesized findings from biochemical, crystallographic, and site-directed mutagenesis studies on integrin heterodimers.
Evaluated minimal peptide recognition motifs and cation-coordination elements, including inserted domains, EF-hand-like loops, and metal ion-dependent adhesion sites.
Integrin family members recognize diverse extracellular matrix proteins, counter-receptors, and microbial ligands primarily through short conserved motifs such as Arg-Gly-Asp (RGD).
Receptor affinity and ligand interactions are tightly coordinated by divalent cations interacting with ligand-competent sites, including the metal ion-dependent adhesion site (MIDAS), alongside allosteric inhibitory calcium sites.
Ligand binding pockets require cooperative contacts from both alpha and beta subunits, or specialized inserted (I) domains containing divalent cation coordination centers.

Cite This Study

Plow et al. (2000) studied this question.

synapsesocial.com/papers/6a6faa94fe4101aa97dfdc37 https://doi.org/10.1074/jbc.r000003200

View Full Paper

Ask AI

Bookmark

Implication

Review uncovers structural mechanisms of ligand recognition by integrin heterodimers, indicating critical coordination by divalent cation binding sites.

Ligand Binding to Integrins

Discussion

Member takes

Implication

Key Points

Cite This Study

Ligand Binding to Integrins

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider