What question did this study set out to answer?

To explore how platelet-derived growth factor (PDGF) influences prostaglandin H synthase (PGHS) mRNA levels and prostaglandin E2 (PGE2) synthesis.

Regulation of Prostaglandin H Synthase mRNA Levels and Prostaglandin Biosynthesis by Platelet-derived Growth Factor

View Full Paper

Ask AI

Bookmark

Authors

Randomized trial shows regulation of prostaglandin biosynthesis in NIH-3T3 cells, suggesting alternate pathways of synthesis.

To explore how platelet-derived growth factor (PDGF) influences prostaglandin H synthase (PGHS) mRNA levels and prostaglandin E2 (PGE2) synthesis.
Stimulation of serum-starved NIH-3T3 cells with rPDGF and measurement of PGE2 synthesis.
Inhibition of translation with cycloheximide to assess the role of PGHS synthesis.
Kinetic analysis of PGHS mRNA levels and immunoblot quantification of PGHS.
rPDGF stimulation led to over 90% inhibition of PGE2 synthesis with cycloheximide.
PGHS mRNA levels increased post-maximal PGE2 synthesis, suggesting a non-dependence on de novo synthesis.
Aspirin treatment synchronized peak PGHS mRNA levels and PGE2 synthesis at 3 h, enhancing PGHS induction.

Lin et al. (1989) studied this question.

View Full Paper

Ask AI

Bookmark