What question did this study set out to answer?

To characterize the submicroscopic structure of the animal cell cytoplasmic ground substance and establish the identity of its basophilic components.

May 1, 1953The Journal of Experimental MedicineOpen Access

Observations on a Submicroscopic Basophilic Component of Cytoplasm

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Authors

KPKeith R. PorterUniversity of Colorado Boulder

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Implication

Ultrastructural study reveals the endoplasmic reticulum across cultured animal cells, indicating its identity with the RNA-rich basophilic ergastoplasm.

Key Points

To characterize the submicroscopic structure of the animal cell cytoplasmic ground substance and establish the identity of its basophilic components.
Examined animal tissue cells cultured in vitro using transmission electron microscopy.
Correlated electron microscope observations with phase contrast and dark field imaging of living cells, as well as light microscopy of cells stained with hematoxylin or toluidine blue.
Identified a universal, membrane-limited reticular network—termed the endoplasmic reticulum—ranging from fine strands (50 to 100 mµ in diameter) to coarse, vesiculated structures (600 mµ in diameter) across all cell types.
Demonstrated that this network corresponds directly to the basophilic ergastoplasm visible by light microscopy and accounts for cytoplasmic regions characterized by high RNA content.

Cite This Study

Keith R. Porter (1953) studied this question.

synapsesocial.com/papers/6a6fad442163a0a01bc3f320 https://doi.org/10.1084/jem.97.5.727

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May 1, 1953The Journal of Experimental MedicineOpen Access

Observations on a Submicroscopic Basophilic Component of Cytoplasm

View Full Paper

Ask AI

Bookmark

Authors

KPKeith R. PorterUniversity of Colorado Boulder

Discussion

Member takes

Implication

Ultrastructural study reveals the endoplasmic reticulum across cultured animal cells, indicating its identity with the RNA-rich basophilic ergastoplasm.

Key Points

To characterize the submicroscopic structure of the animal cell cytoplasmic ground substance and establish the identity of its basophilic components.
Examined animal tissue cells cultured in vitro using transmission electron microscopy.
Correlated electron microscope observations with phase contrast and dark field imaging of living cells, as well as light microscopy of cells stained with hematoxylin or toluidine blue.
Identified a universal, membrane-limited reticular network—termed the endoplasmic reticulum—ranging from fine strands (50 to 100 mµ in diameter) to coarse, vesiculated structures (600 mµ in diameter) across all cell types.
Demonstrated that this network corresponds directly to the basophilic ergastoplasm visible by light microscopy and accounts for cytoplasmic regions characterized by high RNA content.

Cite This Study

Keith R. Porter (1953) studied this question.

synapsesocial.com/papers/6a6fad442163a0a01bc3f320 https://doi.org/10.1084/jem.97.5.727

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