November 1, 1979American Journal of Anatomy
Electron microscopic visualization of the filamentous reticulum in whole cultured presumptive chick myoblasts
Key result
Treatment of cultured chick myoblasts with Triton X-100 and critical-point drying allowed high-resolution 3D visualization of the interconnected filamentous reticulum using electron microscopy.
Population
Cultured presumptive chick myoblasts
Design
Preclinical
Authors
Discussion
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Member takes
Implication
No immediate clinical implications; leaves open validation of cytoskeletal imaging techniques in mammalian cardiac models.
Structured PICO
P
Population
Cultured presumptive chick myoblasts
I
Intervention
Culturing on carbon-coated titanium grids, treatment with Triton X-100 to extract soluble cytoplasm, followed by fixation and critical-point drying
O
Outcome
Visualization and classification of the filamentous reticulum using transmission electron microscopysurrogate
This study describes a novel preparation technique for transmission electron microscopy that enables high-resolution 3D visualization of the filamentous reticulum in whole cultured cells.
Cite This Study
Pudney et al. (1979) studied Cultured presumptive chick myoblasts. Triton X-100 treatment and critical-point drying was evaluated on Visualization of filamentous reticulum. Treatment of cultured chick myoblasts with Triton X-100 and critical-point drying allowed high-resolution 3D visualization of the interconnected filamentous reticulum using electron microscopy.