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November 1, 1996Journal of Medical Virology

Sensitive detection of humanCaliciviridae by RT-PCR

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Why the study?

Does RT-PCR improve the detection of human Caliciviridae compared to direct electron microscopy in faecal samples from patients with gastroenteritis?

Population

166 faecal samples from patients with gastroenteritis sent to the Norwegian National Institute of Public…

Comparison

Semi-nested reverse transcriptase-polymerase… vs Direct electron microscopy (EM)

Design

Cross-sectional

Authors

KSKathrine Stene-JohansenNorwegian Institute of Public HealthBGBjørn GrindeUniversity of Oslo

Discussion

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Implication

RT-PCR may improve Caliciviridae detection in gastroenteritis; leaves open its role versus EM in routine diagnostics.

Structured PICO

Does RT-PCR improve the detection of human Caliciviridae compared to direct electron microscopy in faecal samples from patients with gastroenteritis?

P
Population
166 faecal samples from patients with gastroenteritis sent to the Norwegian National Institute of Public Health for routine diagnosis
I
Intervention
Semi-nested reverse transcriptase-polymerase chain reaction (RT-PCR)
C
Comparator
Direct electron microscopy (EM)
O
Outcome
Detection of human Caliciviridaesurrogate

A newly developed semi-nested RT-PCR method demonstrated higher sensitivity than direct electron microscopy for detecting human Caliciviridae in faecal samples.

Cite This Study

Stene-Johansen et al. (1996) studied this question.

synapsesocial.com/papers/6a6faf2f5d37378ac1dcbdcdhttps://doi.org/10.1002/(sici)1096-9071(199611)50:3<207::aid-jmv1>3.0.co;2-d
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Sequence similarity of human caliciviruses and small round structured viruses1994 · 56 citations
  2. 2Sequence diversity of small, round-structured viruses in the Norwalk virus group1994 · 244 citations
  3. 3A simple procedure for optimising the polymerase chain reaction (PCR) using modified Taguchi methods1994 · 284 citations
  4. 4Aetiological Agent of Enterically Transmitted Non-A, Non-B Hepatitis1988 · 152 citations
  5. 5The electron microscopical and physical characteristics of small round human fecal viruses: An interim scheme for classification1982 · 205 citations