Specificity of dot hybridization assay in the presence of rRNA for detection of rotaviruses in clinical specimens
Why the study?
Is the rotavirus dot hybridization assay specific for detecting rotaviruses in clinical specimens containing eucaryotic rRNA?
Population
95 clinical specimens (extracts of fecal specimens) containing eucaryotic rRNA
Design
Other
Authors
Discussion
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Member takes
Implication
Supports high specificity in limited testing; leaves open larger validation before clinical adoption.
Structured PICO
Is the rotavirus dot hybridization assay specific for detecting rotaviruses in clinical specimens containing eucaryotic rRNA?
P
Population
95 clinical specimens (extracts of fecal specimens) containing eucaryotic rRNA
I
Intervention
Rotavirus dot hybridization assay using a cDNA probe complementary to all 11 rotavirus genes
O
Outcome
Specificity of the rotavirus dot hybridization assaysurrogate
The rotavirus dot hybridization assay is highly specific for detecting rotaviruses even in the presence of eucaryotic rRNA.
Cite This Study
Eiden et al. (1987) studied this question.