October 1, 2001Journal of VirologyOpen Access

Protective Immunity and Antibody-Secreting Cell Responses Elicited by Combined Oral Attenuated Wa Human Rotavirus and Intranasal Wa 2/6-VLPs with MutantEscherichia coliHeat-Labile Toxin in Gnotobiotic Pigs

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Population

Gnotobiotic pigs (near-term pigs derived by hysterectomy and maintained in isolation units), n=64

Comparison

Combined rotavirus vaccination regimens… vs Individual rotavirus vaccine regimens, mLT…

Design

Preclinical

Follow-up

35 days post-initial inoculation (7 days post-challenge)

Authors

LYLijuan YuanBaoding People's Hospital CICristiana IosefChildren’s Health Research Institute MAMarli S.P. AzevedoNational Center for Toxicological Research

Discussion

Member takes

Implication

Should not change rotavirus vaccination practice; extends preclinical mucosal immunity data in pigs, warranting human trials.

Structured PICO

Population

Gnotobiotic pigs (near-term pigs derived by hysterectomy and maintained in isolation units), n=64

Intervention

Combined rotavirus vaccination regimens: AttHRV/VLP2x (one oral dose of attenuated Wa HRV followed by two intranasal doses of 2/6-VLPs + mLT) or VLP2x/AttHRV (two i.n. doses of 2/6-VLPs + mLT followed by one oral dose of attenuated Wa HRV)

Comparator

Individual rotavirus vaccine regimens (1 or 3 oral doses of attenuated Wa HRV, 3 i.n. doses of 2/6-VLPs + mLT, 3 i.n. doses of inactivated Wa HRV + mLT), mLT alone, or mock-inoculated

Outcome

Protection against virus shedding and diarrhea upon challenge with virulent Wa HRV, and rotavirus-specific antibody-secreting cell (ASC) responsessurrogate

A combined oral live-attenuated and intranasal VLP rotavirus vaccination regimen effectively stimulates mucosal immunity and protects against rotavirus disease in a gnotobiotic pig model.

Limitations

Small numbers of pigs in some groups requiring pooling of data from previous studies

Cite This Study

Yuan et al. (2001) studied this question.

synapsesocial.com/papers/6a6fb311439bab0cabc2e1c9 https://doi.org/10.1128/jvi.75.19.9229-9238.2001

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Protective Immunity and Antibody-Secreting Cell Responses Elicited by Combined Oral Attenuated Wa Human Rotavirus and Intranasal Wa 2/6-VLPs with MutantEscherichia coliHeat-Labile Toxin in Gnotobiotic Pigs

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Protective Immunity and Antibody-Secreting Cell Responses Elicited by Combined Oral Attenuated Wa Human Rotavirus and Intranasal Wa 2/6-VLPs with MutantEscherichia coliHeat-Labile Toxin in Gnotobiotic Pigs

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider