What question did this study set out to answer?

To determine whether glucose metabolism through the pentose cycle pathway mediates glucose-stimulated insulin secretion in mouse pancreatic islets.

February 1, 1972Biochemical JournalOpen Access

The pentose cycle and insulin release in mouse pancreatic islets

View Full Paper

Ask AI

Bookmark

Authors

SAS. J. H. AshcroftUniversity of Copenhagen LWLaksiri WeerasingheUniversity of Sri Jayewardenepura JBJ. M. BassettSalford Royal NHS Foundation Trust

Discussion

Member takes

Implication

In vitro experiment demonstrates that pentose cycle oxidation does not track insulin secretion in mouse pancreatic islets, indicating the pathway does not drive glucose-stimulated release.

Key Points

To determine whether glucose metabolism through the pentose cycle pathway mediates glucose-stimulated insulin secretion in mouse pancreatic islets.
Incubated isolated mouse pancreatic islets in vitro with radiolabeled glucose substrates to quantify glucose utilization ([3H]water formation) and oxidation (14CO2 formation).
Measured rates of insulin release and pentose cycle flux across glucose concentrations from 3.3 to 16.7 mM, with and without caffeine (5 mM).
Assayed the effects of metabolic inhibitors, secretagogues, and pentose intermediates (ribose, xylitol, and ribitol) on islet insulin release and glucose oxidation.
Increasing glucose concentration from 3.3 to 16.7 mM stimulated insulin secretion 5-fold (30-fold in the presence of 5 mM caffeine) and increased glucose utilization, but pentose cycle glucose oxidation showed no increase.
Glucosamine (20 mM) inhibited glucose utilization and insulin release without altering pentose cycle flux, whereas phenazine methosulphate (5 µM) accelerated pentose cycle flux while inhibiting insulin secretion.
Ribose, xylitol, and ribitol failed to stimulate insulin release or alter glucose oxidation and utilization, producing negligible 14CO2 formation.

Cite This Study

Ashcroft et al. (1972) studied this question.

synapsesocial.com/papers/6a6fbbbc660549caf2c446b3 https://doi.org/10.1042/bj1260525

View Full Paper

Ask AI

Bookmark

Implication

In vitro experiment demonstrates that pentose cycle oxidation does not track insulin secretion in mouse pancreatic islets, indicating the pathway does not drive glucose-stimulated release.

The pentose cycle and insulin release in mouse pancreatic islets

Discussion

Member takes

Implication

Key Points

Cite This Study

The pentose cycle and insulin release in mouse pancreatic islets

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider