What question did this study set out to answer?

To determine whether transplanting double-negative T cells (DNTαβ) modulates the local immune microenvironment and enhances neuroprotection following traumatic spinal cord injury.

September 23, 2025NeurotherapeuticsOpen Access

Neuroprotective role of DNTαβ Cell transplantation on modulating immune microenvironment in spinal cord injured mice

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Authors

YJYingli JingCapital Medical University ZLZihan LiNational Health and Family Planning Commission FBFan BaiHunan University of Traditional Chinese Medicine

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Implication

Preclinical study demonstrates that DNTαβ cell transplantation restores motor function in spinal cord-injured mice, highlighting a novel cell-based therapy.

Key Points

To determine whether transplanting double-negative T cells (DNTαβ) modulates the local immune microenvironment and enhances neuroprotection following traumatic spinal cord injury.
Sorted CD3+ TCRαβ+ CD4- CD8- NK1.1- T cells (DNTαβ) using flow cytometry after identifying double-negative T cell dynamics and heterogeneity via single-cell sequencing.
Administered isolated DNTαβ cells via in situ local injection or systemic tail vein injection into mice with spinal cord injury.
Evaluated motor recovery through behavioral assessments, tissue repair via immunohistochemistry, and underlying mechanisms using transcriptomic sequencing.
Both in situ and tail vein injections of DNTαβ cells significantly promoted motor function recovery in mice following spinal cord injury.
Immunohistochemical staining demonstrated that DNTαβ cell transplantation inhibited glial proliferation, increased neuronal survival, and promoted tissue myelination.
Transcriptomic profiling showed that DNTαβ cells regulated early repair and neuroprotection primarily by modulating immune responses via the Gzmb and IFN pathways.

Cite This Study

Jing et al. (2025) studied this question.

synapsesocial.com/papers/6a6fbd07e71d69abee082f21 https://doi.org/10.1016/j.neurot.2025.e00743

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Implication

Preclinical study demonstrates that DNTαβ cell transplantation restores motor function in spinal cord-injured mice, highlighting a novel cell-based therapy.

Neuroprotective role of DNTαβ Cell transplantation on modulating immune microenvironment in spinal cord injured mice

Discussion

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Implication

Key Points

Cite This Study

Neuroprotective role of DNTαβ Cell transplantation on modulating immune microenvironment in spinal cord injured mice

Discussion

Member takes

Implication

Key Points

Cite This Study

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