In vivo trials, utilizing four fistulated steers in a Latin-square design~ were conducted with four rations: casein + basal, urea + basal, soybean meal basal, and cottonseed hulls (basal). Nitrogenous constituents, volatile fatty acids ( V F A ) , and p i t values were determined on rumen liquor taken at various intervals after feeding. Levels of urea nitrogen were determined on the jugular blood.
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Davis et al. (1964) studied this question.
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