Recent advances in single-molecule enzymology are reviewed. The theoretical underpinning of sincle-molecule enzymatic behaviors is discussed and exemplified by experiements and statistical analyses. In particular, the manifestations of the Michaelis-Menten mechanism, the kinetic scheme with sequential intermediates, and dynamic disorder in single molecule data are presented. A survey of current methods for single molecule enzymatic assays, especially those based on fluorescence detection, is presented as well.
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Sunney Xie (2001) studied this question.