Quantum efficiency of the photoreaction with photoproduct filtering effect and application to light-adaptation of purple membrane

In order to estimate the quantum efficiency of the photoreaction involving the filtering effect due to the photoproduct, the new equation for analyzing a photoreaction was devised: The filtering effects due to not only the reactant but also the photoproduct were considered in the method. As an application of this equation, we estimated the quantum efficiency of light-adaptation of purple membrane (PM) at 0°C. It was concluded that the quantum efficiency for this reaction was independent of the irradiation wavelengths and the value was 0.14 ± 0.01.