July 1, 2002Journal of Biological ChemistryOpen Access

Actin-induced Closure of the Actin-binding Cleft of Smooth Muscle Myosin

View Full Paper

Ask AI

Bookmark

Population

Genetically engineered truncated version of smooth muscle myosin containing the motor domain and the…

Design

Preclinical

Authors

CYChristopher M. YengoPennsylvania State University ECEnrique M. De La CruzYale University LCLynn R. ChrinUniversity of Vermont

Discussion

Member takes

Implication

Elucidates myosin-actin regulation in smooth muscle; leaves open cardiac isoform relevance and in vivo translation.

Structured PICO

Population

Genetically engineered truncated version of smooth muscle myosin containing the motor domain and the essential light chain-binding region (MDE), with a single tryptophan residue at position 425 (F425W-MDE)

Intervention

Exposure to MgATP, MgADP, or absence of nucleotide

Outcome

Conformation of Trp-425 in the actin-binding cleft and rates of strong actin bindingsurrogate

Actin induces conformational changes in the actin-binding cleft of smooth muscle myosin that are required to generate force and motion during muscle contraction.

Cite This Study

Yengo et al. (2002) studied this question.

synapsesocial.com/papers/6a6fc6116ceb2bbd16dfba07 https://doi.org/10.1074/jbc.m111253200

View Full Paper

Ask AI

Bookmark

Actin-induced Closure of the Actin-binding Cleft of Smooth Muscle Myosin

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Actin-induced Closure of the Actin-binding Cleft of Smooth Muscle Myosin

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider