What question did this study set out to answer?

To determine whether physiological challenges that trigger corticotropin-releasing factor, oxytocin, and vasopressin alter NGFI-B and c-fos expression in stress-related neurosecretory neurons.

December 1, 1993Journal of NeuroscienceOpen Access

A comparison of two immediate-early genes, c-fos and NGFI-B, as markers for functional activation in stress-related neuroendocrine circuitry

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Authors

RCRK ChanSalk Institute for Biological Studies EBER BrownSalk Institute for Biological Studies AEAnders EricssonLund University

Discussion

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Implication

Animal experiment demonstrates induction of NGFI-B alongside c-fos in neurosecretory neurons, indicating NGFI-B's utility as an activation marker.

Key Points

To determine whether physiological challenges that trigger corticotropin-releasing factor, oxytocin, and vasopressin alter NGFI-B and c-fos expression in stress-related neurosecretory neurons.
Used combined immunohistochemistry and in situ hybridization histochemistry in rats.
Administered four stress challenges: acute hypotensive hemorrhage (15% blood volume reduction), intravenous interleukin-1 beta (1.87 µg/kg), chronic salt loading (7 days on 2% saline), and acute bilateral adrenalectomy.
Unperturbed controls displayed constitutively high NGFI-B mRNA in the telencephalon but not the endocrine hypothalamus, whereas basal c-fos expression remained uniformly low across the CNS.
Both NGFI-B and c-fos mRNAs, along with Fos protein, were induced across parallel time courses and in similar neurosecretory cell types across all four stress models.
c-fos was readily detectable in mediating extrahypothalamic afferent pathways (including medullary catecholaminergic neurons and lamina terminalis groups), whereas NGFI-B displayed lower detection sensitivity in these afferent circuits.

Cite This Study

Chan et al. (1993) studied this question.

synapsesocial.com/papers/6a6fc640f44fa9f079dd43de https://doi.org/10.1523/jneurosci.13-12-05126.1993

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December 1, 1993Journal of NeuroscienceOpen Access

A comparison of two immediate-early genes, c-fos and NGFI-B, as markers for functional activation in stress-related neuroendocrine circuitry

View Full Paper

Ask AI

Bookmark

Authors

RCRK ChanSalk Institute for Biological Studies EBER BrownSalk Institute for Biological Studies AEAnders EricssonLund University

Discussion

Member takes

Implication

Animal experiment demonstrates induction of NGFI-B alongside c-fos in neurosecretory neurons, indicating NGFI-B's utility as an activation marker.

Key Points

To determine whether physiological challenges that trigger corticotropin-releasing factor, oxytocin, and vasopressin alter NGFI-B and c-fos expression in stress-related neurosecretory neurons.
Used combined immunohistochemistry and in situ hybridization histochemistry in rats.
Administered four stress challenges: acute hypotensive hemorrhage (15% blood volume reduction), intravenous interleukin-1 beta (1.87 µg/kg), chronic salt loading (7 days on 2% saline), and acute bilateral adrenalectomy.
Unperturbed controls displayed constitutively high NGFI-B mRNA in the telencephalon but not the endocrine hypothalamus, whereas basal c-fos expression remained uniformly low across the CNS.
Both NGFI-B and c-fos mRNAs, along with Fos protein, were induced across parallel time courses and in similar neurosecretory cell types across all four stress models.
c-fos was readily detectable in mediating extrahypothalamic afferent pathways (including medullary catecholaminergic neurons and lamina terminalis groups), whereas NGFI-B displayed lower detection sensitivity in these afferent circuits.

Cite This Study

Chan et al. (1993) studied this question.

synapsesocial.com/papers/6a6fc640f44fa9f079dd43de https://doi.org/10.1523/jneurosci.13-12-05126.1993

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