January 26, 1986Nucleic Acids ResearchOpen Access

Promoter and enhancer activities of long terminal repeats associated with cellular retrovirus-like (VL30) elements

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Population

Mouse L-cells, monkey CV-1 cells, and monkey COS cells

Comparison

Transfection with VL30 LTR-containing DNA… vs Transfection with constructs containing MSV LTR…

Design

Preclinical

Authors

Discussion

Cellular LTRs may exceed viral promoter strength in preclinical models; leaves open their regulatory roles in endogenous retroelements.

Population

Mouse L-cells, monkey CV-1 cells, and monkey COS cells

Intervention

Transfection with VL30 LTR-containing DNA constructs (e.g., VL3 LTR-pSVOCAT)

Comparator

Transfection with constructs containing MSV LTR, SV40 early promoter (pSV2CAT), or mock transfection

Outcome

Promoter and enhancer activities measured by chloramphenicol acetyl transferase (CAT) expressionsurrogate

Certain resident cellular LTRs (like VL3) possess promoter and enhancer capacities greater than those of infectious retroviruses.

Rotman et al. (1986) studied this question.

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