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The survival and the lipid composition of mussel ( Mytilus trossulus ) larval cells was analyzed before and after cryopreservation in two protective media. Cell survival and functional recovery were greatly improved when the conventional DMSO + trehalose were supplemented with a lipid extract from mature mollusks ( Crenomytilus grayanus ) + vitamins E and C. These additives also markedly influenced the fatty acid composition of the cells upon thawing, particularly as regards the share of monoenic acids and the unsaturation index. The probable causes of these effects are discussed.
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Kostetsky et al. (2008) studied this question.
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