August 20, 2013Journal of Biological ChemistryOpen Access

Metal Switch-controlled Myosin II from Dictyostelium discoideum Supports Closure of Nucleotide Pocket during ATP Binding Coupled to Detachment from Actin Filaments

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Population

Wild-type and the S237C mutant of non-muscle myosin II motor from Dictyostelium discoideum

Comparison

S237C substitution and exposure to different… vs Wild-type non-muscle myosin II motor

Design

Preclinical

Authors

JCJared C. CochranIndiana University Bloomington MTMorgan E. Thompson FKF. Jon KullDartmouth College

Discussion

Member takes

Implication

S237C myosin enables study of actin-nucleotide linkage in vitro; leaves open extension to cardiac models.

Structured PICO

Population

Wild-type and the S237C mutant of non-muscle myosin II motor from Dictyostelium discoideum

Intervention

S237C substitution and exposure to different divalent metals (Mg2+ or Mn2+)

Comparator

Wild-type non-muscle myosin II motor

Outcome

ATPase kinetics and actin-binding interactionssurrogate

The S237C substitution in myosin II switch-1 provides a reversible structural linkage between the nucleotide pocket and actin-binding region that is sensitive to manganese.

Cite This Study

Cochran et al. (2013) studied this question.

synapsesocial.com/papers/6a6fd37dc1a24a6142db665c https://doi.org/10.1074/jbc.m113.466045

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