What question did this study set out to answer?

To determine whether standard phenol-extracted bacterial lipopolysaccharides behave identically to native unextracted forms in biological activity, lipoprotein binding, and in vivo disposition.

October 1, 1982Journal of Clinical InvestigationOpen Access

Biological Activity, Lipoprotein-binding Behavior, and In Vivo Disposition of Extracted and Native Forms of Salmonella typhimurium Lipopolysaccharides

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Authors

RMRobert S. MunfordNational Institutes of Health CHCatherine L. HallUniversity of London JLJ. M. LiptonTexas Medical Center

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Implication

Animal study demonstrates shed and extracted Salmonella lipopolysaccharides bind high-density lipoproteins and prolong plasma clearance in rats, indicating extracts reliably mimic shed endotoxins.

Key Points

To determine whether standard phenol-extracted bacterial lipopolysaccharides behave identically to native unextracted forms in biological activity, lipoprotein binding, and in vivo disposition.
Prepared [3H]galactose-labeled Salmonella typhimurium as whole bacteria, isolated outer membranes, culture supernatant membrane fragments, and phenol-water extracts.
Assayed biological potency via the Limulus amebocyte lysate assay and rabbit pyrogen test, alongside in vitro rat lipoprotein-binding experiments.
Administered preparations intravenously into rats to quantify plasma lipopolysaccharide-HDL binding and 10-minute tissue clearance and distribution.
Phenol-extracted lipopolysaccharides exhibited slightly higher potency in Limulus lysate and rabbit pyrogen tests than native membrane fragments.
Less than 25% of lipopolysaccharides from whole bacteria and outer membranes bound HDL in vitro, resulting in rapid in vivo clearance into the liver and spleen.
Over 50% of lipopolysaccharides from phenol extracts and shed membrane fragments bound HDL in vitro, with approximately 50% remaining in rat plasma over 10 minutes and accumulating in steroidogenic tissues (adrenal glands and ovaries).

Cite This Study

Munford et al. (1982) studied this question.

synapsesocial.com/papers/6a6fdd1cc92390ac2d07ac5b https://doi.org/10.1172/jci110684

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Implication

Animal study demonstrates shed and extracted Salmonella lipopolysaccharides bind high-density lipoproteins and prolong plasma clearance in rats, indicating extracts reliably mimic shed endotoxins.

Biological Activity, Lipoprotein-binding Behavior, and In Vivo Disposition of Extracted and Native Forms of Salmonella typhimurium Lipopolysaccharides

Discussion

Member takes

Implication

Key Points

Cite This Study

Biological Activity, Lipoprotein-binding Behavior, and In Vivo Disposition of Extracted and Native Forms of Salmonella typhimurium Lipopolysaccharides

Discussion

Member takes

Implication

Key Points

Cite This Study

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