What question did this study set out to answer?

To establish and describe a high-resolution intracellular autoradiography method for localizing radiolabeled molecules within cells using electron microscopy.

August 1, 1961The Journal of Cell BiologyOpen Access

A Method for Intracellular Autoradiography in the Electron Microscope

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Authors

MSM. H. SilkUniversity of Cape Town ÅHÅ.O. HawtreyCouncil for Scientific and Industrial Research ISI. M. SpencePoliomyelitis Research Foundation

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Implication

Methodological study demonstrates high-resolution intracellular autoradiography in cultured monkey kidney cells, suggesting cytoplasmic synthesis of thymidine-containing polynucleotides.

Key Points

To establish and describe a high-resolution intracellular autoradiography method for localizing radiolabeled molecules within cells using electron microscopy.
Cultured LLC-MK(2) monkey kidney cells were incubated with 0.4 µCi/ml thymidine-H(3) for 72 hours, fixed with osmium tetroxide, and embedded in methacrylate.
Ultrathin tissue sections on carbon-stabilized grids were coated with 150–450 Å of evaporated silver, exposed to bromine vapor, and overlaid with a 1% aqueous gelatin film.
Grids were exposed for 50 days at 4°C with desiccant, developed in Promicrol, fixed in sodium thiosulphate, cleared of gelatin at 37°C, and imaged by electron microscopy.
Treated cells retained well-defined ultrastructural detail following photochemical processing and gelatin stripping.
Cytoplasm of labeled cells displayed dense, electron-opaque silver deposits, indicating that thymidine-containing polynucleotides may be synthesized within the cytoplasm.

Cite This Study

Silk et al. (1961) studied this question.

synapsesocial.com/papers/6a6fe2748031ec7bb1dbfd51 https://doi.org/10.1083/jcb.10.4.577

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Implication

Methodological study demonstrates high-resolution intracellular autoradiography in cultured monkey kidney cells, suggesting cytoplasmic synthesis of thymidine-containing polynucleotides.

A Method for Intracellular Autoradiography in the Electron Microscope

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Implication

Key Points

Cite This Study

A Method for Intracellular Autoradiography in the Electron Microscope

Discussion

Member takes

Implication

Key Points

Cite This Study

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