What question did this study set out to answer?

To develop a high-speed electron microscope autoradiography technique that dramatically reduces exposure time when imaging diffusible radioisotope-labeled compounds.

January 1, 1981Journal of Histochemistry & CytochemistryOpen Access

High-Speed Electron Microscope Autoradiographic Studies of Diffusible Compounds,

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Authors

VMVinci MizuhiraTsurumi University MSMichiko Shiihashi YFYutaka FutaesakuKitasato University

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Implication

Experimental study demonstrates a 100-fold sensitivity increase in tissue autoradiography, indicating exposure time can drop from one month to four days.

Key Points

To develop a high-speed electron microscope autoradiography technique that dramatically reduces exposure time when imaging diffusible radioisotope-labeled compounds.
Assessed radioisotope incorporation and tissue fixation using labeled compounds including thymidine, 3H-SPG, and iodine.
Applied photographic emulsion to both sides of the specimen grid combined with an overdevelopment chemical processing protocol.
Achieved an approximate 100-fold increase in sensitivity compared with conventional electron microscope autoradiography.
Reduced the required autoradiography exposure time from four weeks or more down to four days.

Cite This Study

Mizuhira et al. (1981) studied this question.

synapsesocial.com/papers/6a880f8cbc5f9fd513bbf2c3 https://doi.org/10.1177/29.1a_suppl.7288151

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Implication

Experimental study demonstrates a 100-fold sensitivity increase in tissue autoradiography, indicating exposure time can drop from one month to four days.

High-Speed Electron Microscope Autoradiographic Studies of Diffusible Compounds,

Discussion

Member takes

Implication

Key Points

Cite This Study

High-Speed Electron Microscope Autoradiographic Studies of Diffusible Compounds,

Discussion

Member takes

Implication

Key Points

Cite This Study

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