Experimental method demonstrates accelerated autoradiographic labeling using liquid scintillator at low temperatures, suggesting significant time savings for biological sample processing.
Impregnation of nuclear track emulsion with liquid scintillator and exposure at -85 degrees C allows rapid autoradiographic labeling. With tritiated thymidine of high specific activity (40 to 60 curies per millimole), exposure time can be shortened to 20 to 60 minutes, allowing complete sample processing within 4 hours. In experiments requiring isotopes with low incorporation rates or low specific activity, exposure time can be shortened from months to several days.
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Durie et al. (1975) studied this question.
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