Purification and kinetics study of thermostable cellulase free Xylanase from Bacillus subtilis

This is the authors' abstract. We don't add key points for this paper.

Present study dealt with the production, purification and characterization of xylanase from Bacillus subtilis- BS05 grown in submerged fermentation at 37(o)C for 48h using sugarcane bagasse as a substrate. Xylanase enzyme was purified to homogeneity by ammonium sulphate fractionation followed by sephadex G-100 chromatography. The molecular mass of xylanase enzyme was found to be 23kDa by SDS-PAGE. The optimum pH and temperature of xylanase enzyme was 5 and 50°C with stability range of 5-6 and 30°C -50°C respectively. The enzyme had half life of 1386-1155 minutes at 30°C -50°C respectively. Metal profile of the enzyme showed that Mn(2+) (127.4 %) and Fe(2+) (115%) were the activators while Hg(2+) (14%) and EDTA (19%) were the inhibitors. Kinetic parameters revealed that the enzyme is specific to birchwood xylan with Km , Kcat , Vmax and Kcat/Km value of 1.15 mg/ml, 850 s(-1), 117.64 U/mg and 739.13 s(-1)mg(-1).mL respectively.