Optimization of xylanase production from Enterobacter asburiae PQ396173 for hydrolysis of sugarcane bagasse

The objective of this study was to identify effective microbial strains from the Similipal Biosphere Reserve that could hydrolyse sugarcane bagasse by producing the xylanase enzyme. The most effective bacterial strain, SSXB1, had the greatest enzyme activity of 191.86 U/ mL /min in an enzyme assay for xylanase activity under unoptimised circumstances. The Box-Behnken design of response surface approach was used in this study to maximise xylanase output from SSXB1 by optimising the culture conditions. The enzyme activity reached 578.13 U /mL /min in a procedure with optimal parameters, including temperature 35.0 °C, pH 7.5, xylan content 3%, and incubation duration of 36 h. Using biochemical and molecular techniques, the bacterial strain SSXB1 was determined to be Enterobacter asburiae PQ396173. Using E. asburiae PQ396173 xylanase, which demonstrated a considerable quantity of reducing sugar release from pretreatment biomass under optimal circumstances; further enzymatic hydrolysis of sodium carbonate pretreated sugarcane baggase was optimized. Furthermore, structural changes in the pretreated biomass were discovered by scanning electron microscopy (SEM) examination of sugarcane baggase that had been left untreated, pretreated with sodium carbonate, pretreated with commercial enzyme (BG-xylan), and pretreated with E. asburiae PQ396173 xylanase. This work is the first report on xylanase production from E. asburiae PQ396173.