March 19, 2009AJP Cell PhysiologyOpen Access

CapZ dynamics are altered by endothelin-1 and phenylephrine via PIP2- and PKC-dependent mechanisms

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Structured PICO

Population

Cultured neonatal rat ventricular myocytes (NRVM) isolated from 1- to 2-day-old neonatal Sprague-Dawley rats, infected with adenovirus expressing green fluorescent protein-CapZ beta1.

Intervention

Endothelin-1 (100 nM) for 4 hours or phenylephrine (10 μM) for 24 hours, with or without PIP2 sequestration (neomycin 500 μM) or PKC inhibition (chelerythrine chloride 10 μM).

Comparator

Untreated control myocytes expressing GFP-CapZ.

Outcome

CapZ dynamics measured by fluorescence recovery after photobleaching (FRAP) recovery percentage at 30 minutes postbleach.surrogate

Endothelin and phenylephrine alter CapZ dynamics through PIP2- and PKC-dependent pathways, which may destabilize the existing sarcomeric framework and permit remodeling during cardiac hypertrophy.

Limitations

Addition of PE or ET to low-density NRVM cultures can elicit calcium oscillations and spontaneous contractile activity, which may have directly or indirectly contributed to the alteration in CapZ dynamics.

Discussion

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Implication

May inform hypertrophy remodeling mechanisms; hypothesis-generating for PIP2/PKC-targeted therapies pending in vivo human data.

Cite This Study

Hartman et al. (2009) studied this question.

synapsesocial.com/papers/6a6fea70ce524a4339c3bda4 https://doi.org/10.1152/ajpcell.00544.2008

View Full Paper

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CapZ dynamics are altered by endothelin-1 and phenylephrine via PIP2- and PKC-dependent mechanisms

Structured PICO

Limitations

Discussion

Implication

Cite This Study

CapZ dynamics are altered by endothelin-1 and phenylephrine via PIP2- and PKC-dependent mechanisms

Structured PICO

Limitations

Discussion

Implication

Cite This Study

Also Consider

Also Consider