What question did this study set out to answer?

Determine the transport kinetics and molecular mechanism governing intra- and extracellular chloride exchange in human promyelocytic leukemic HL-60 cells.

September 1, 1989AJP Cell Physiology

Cl-Cl exchange in promyelocytic HL-60 cells follows simultaneous rather than ping-pong kinetics

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Determine the transport kinetics and molecular mechanism governing intra- and extracellular chloride exchange in human promyelocytic leukemic HL-60 cells.
Measured radioactive isotope (36Cl) efflux over time under varied intra- and extracellular chloride concentrations in human promyelocytic leukemic HL-60 cells.
Analyzed exchange flux relationships, maximum velocities (Vmax), and apparent Michaelis constants (Km) as a function of trans-chloride concentrations.
Radioactive isotope efflux followed an exponential time course, with Cl-Cl exchange conforming to Michaelis-Menten kinetics for both intra- and extracellular chloride.
The Vmax to apparent Km ratio for intra- and extracellular substrates increased with trans-chloride concentration, refuting ping-pong kinetics.
Observed exchange rates matched a kinetic model where intra- and extracellular chloride ions bind in random order and translocate simultaneously.

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Implication

Kinetic analysis reveals simultaneous chloride ion translocation during Cl-Cl exchange in promyelocytic HL-60 cells, indicating random-order substrate binding over ping-pong mechanisms.

Cite This Study

Restrepo et al. (1989) studied this question.

synapsesocial.com/papers/6a6ff42b78a11c550e09bcca https://doi.org/10.1152/ajpcell.1989.257.3.c520

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Implication

Kinetic analysis reveals simultaneous chloride ion translocation during Cl-Cl exchange in promyelocytic HL-60 cells, indicating random-order substrate binding over ping-pong mechanisms.

Cl-Cl exchange in promyelocytic HL-60 cells follows simultaneous rather than ping-pong kinetics

Key Points

Discussion

Implication

Cite This Study

Cl-Cl exchange in promyelocytic HL-60 cells follows simultaneous rather than ping-pong kinetics

Key Points

Discussion

Implication

Cite This Study

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