November 1, 1991Biochemistry

The two calcium ions initially bound to nonphosphorylated sarcoplasmic reticulum calcium ATPase can no longer be kinetically distinguished when they dissociate from phosphorylated ATPase toward the lumen

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Population

Sarcoplasmic reticulum vesicles made leaky with an ionophore

Comparison

ATP-induced phosphorylation under various pH and… vs Nonphosphorylated ATPase (absence of ATP)

Design

Preclinical

Authors

SOStéphane OrlowskiCentre National de la Recherche Scientifique PCPhilippe ChampeilUniversité Paris-Sud

Discussion

Member takes

Implication

Equal Ca²⁺ dissociation kinetics after SERCA phosphorylation extends mechanistic models; leaves open relevance to human cardiac calcium handling.

Structured PICO

Population

Sarcoplasmic reticulum vesicles made leaky with an ionophore

Intervention

ATP-induced phosphorylation under various pH and ionic conditions

Comparator

Nonphosphorylated ATPase (absence of ATP)

Outcome

Kinetics of 45Ca2+ dissociation/release toward the lumensurrogate

The study demonstrates that the two calcium ions bound to sarcoplasmic reticulum calcium ATPase become kinetically indistinguishable upon ATP-induced phosphorylation when dissociating toward the lumen.

Cite This Study

Orlowski et al. (1991) studied this question.

synapsesocial.com/papers/6a6ff94bfebe604dd708c9d8 https://doi.org/10.1021/bi00111a020

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Implication

Equal Ca²⁺ dissociation kinetics after SERCA phosphorylation extends mechanistic models; leaves open relevance to human cardiac calcium handling.

The two calcium ions initially bound to nonphosphorylated sarcoplasmic reticulum calcium ATPase can no longer be kinetically distinguished when they dissociate from phosphorylated ATPase toward the lumen

Discussion

Member takes

Implication

Structured PICO

Cite This Study

The two calcium ions initially bound to nonphosphorylated sarcoplasmic reticulum calcium ATPase can no longer be kinetically distinguished when they dissociate from phosphorylated ATPase toward the lumen

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider