January 15, 1991Biochemistry

Kinetics of calcium dissociation from its high-affinity transport sites on sarcoplasmic reticulum ATPase

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Population

Sarcoplasmic reticulum Ca2(+)-ATPase

Design

Preclinical

Authors

SOStéphane OrlowskiCentre National de la Recherche Scientifique PCPhilippe ChampeilUniversité Paris-Sud

Discussion

Member takes

Implication

Sequential SR ATPase kinetics inform Ca2+ pump models; leaves open whether gating can be targeted in cardiac disease.

Structured PICO

Population

Sarcoplasmic reticulum Ca2(+)-ATPase

Intervention

Fast filtration combined with stopped-flow fluorescence measurements under various conditions (pH 6-7, various temperatures, presence of MgCl2, EGTA, calcium ionophore, KCl, DMSO, 4-nonylphenol, or nucleotide analogue)

Outcome

Kinetics of calcium dissociation from high-affinity transport sitessurrogate

The study demonstrates that calcium dissociation from sarcoplasmic reticulum ATPase occurs sequentially from a narrow binding pocket, potentially controlled by a structural gate.

Cite This Study

Orlowski et al. (1991) studied this question.

synapsesocial.com/papers/6a6ff94bfebe604dd708c9da https://doi.org/10.1021/bi00216a007

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