What question did this study set out to answer?

To evaluate how nebulizer type, saline concentration, and bronchodilator pretreatment influence the success rate, cell yield, and tolerability of sputum induction.

April 1, 1995European Respiratory JournalOpen Access

Some technical factors influencing the induction of sputum for cell analysis

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Authors

TPTodor A. PopovUniversity Hospital St. Ivan Rilski MPMM PizzichiniSt. Joseph Hospital EPEmílio PizzichiniNorthwestern University

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Implication

Randomized crossover trial reveals that hypertonic saline improves sputum induction success over normal saline in asthmatic and healthy adults, indicating technical trade-offs with subject discomfort.

Key Points

To evaluate how nebulizer type, saline concentration, and bronchodilator pretreatment influence the success rate, cell yield, and tolerability of sputum induction.
Conducted three randomized, cross-over trials in 26 asthmatic and 13 healthy subjects unable to produce sputum spontaneously.
Tested nebulizer types (two ultrasonic vs. one jet; n=15), saline concentrations (0.9% vs. 3% vs. sequential 3–5%; n=14, double-blind), and salbutamol versus placebo pretreatment (n=10, double-blind).
Dispersed sputum with dithiothreitol to determine total and differential cell counts while tracking percentage fall in FEV1 and subject discomfort.
Two ultrasonic nebulizers showed no difference in success rates or cell counts, but subject discomfort increased proportionally with higher nebulizer saline output.
Hypertonic saline induction achieved greater success than 0.9% normal saline but caused more discomfort, with 3% saline succeeding in 6 of 14 subjects.

Cite This Study

Popov et al. (1995) studied this question.

synapsesocial.com/papers/6a6ff9c8e71d69abee084e96 https://doi.org/10.1183/09031936.95.08040559

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Some technical factors influencing the induction of sputum for cell analysis

Discussion

Member takes

Implication

Key Points

Cite This Study

Some technical factors influencing the induction of sputum for cell analysis

Discussion

Member takes

Implication

Key Points

Cite This Study

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