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To investigate the global gene expression response of Saccharomyces cerevisiae to alkylating agents.

Global response of Saccharomyces cerevisiae to an alkylating agent

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To investigate the global gene expression response of Saccharomyces cerevisiae to alkylating agents.
Utilized DNA chip technology to analyze transcript levels of approximately 6,200 genes.
Analyzed responses to methyl methanesulfonate exposure, identifying responsive and nonresponsive ORFs.
Conducted conventional Northern blotting for validation of gene expression results.
Approximately 325 gene transcripts increased and 76 decreased following exposure to the alkylating agent.
Of the 21 known DNA-damage inducible genes, 18 were confirmed induced, with new genes showing stronger induction.
Correlation coefficient of 0.79 between DNA chip and Northern blot analysis for gene responsiveness.

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Randomized trial shows gene responses to an alkylating agent in Saccharomyces cerevisiae, highlighting unexpected gene behaviors.

Jelinsky et al. (1999) studied this question.

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