What question did this study set out to answer?

To examine the protective properties of octanoic acid and decanoic acid against tunicamycin-induced endoplasmic reticulum stress in rat aortic smooth muscle cells.

January 1, 2024Advances in Pharmacological and Pharmaceutical SciencesOpen Access

Octanoic Acid and Decanoic Acid Inhibit Tunicamycin‐Induced ER Stress in Rat Aortic Smooth Muscle Cells

View Full Paper

Ask AI

Bookmark

Authors

WSWanida SukketsiriPrince of Songkla University VTVaromyalin TipmaneePrince of Songkla University PRPanlekha RungruangMinistry of Public Health

Discussion

Member takes

Implication

In vitro study demonstrates that octanoic and decanoic acids reduce tunicamycin-induced cellular stress in rat aortic smooth muscle cells, indicating a potential protective therapy for vascular...

Key Points

To examine the protective properties of octanoic acid and decanoic acid against tunicamycin-induced endoplasmic reticulum stress in rat aortic smooth muscle cells.
Pretreated rat aortic smooth muscle cells with octanoic acid or decanoic acid at concentrations of 250 μM and 500 μM for 24 hours, followed by exposure to 1 μg/mL tunicamycin for 24 hours.
Evaluated apoptosis using DAPI staining and quantified intracellular reactive oxygen species via DCFH2-DA fluorescence.
Assessed gene expression of ER stress markers (CHOP, GRP78, ATF4, eIF2α) and contractile markers (αSMA, MYH11) using RT-qPCR, and measured αSMA protein using immunocytochemistry.
Pretreatment with octanoic acid and decanoic acid significantly mitigated tunicamycin-induced cell death and reduced reactive oxygen species generation.
Both fatty acids inhibited the gene expression of endoplasmic reticulum stress markers, including CHOP, GRP78, and eIF2α.
Octanoic acid and decanoic acid reduced contractile gene expression of α-SMA and MYH11 and lowered the count of α-SMA-positive cells.

Cite This Study

Sukketsiri et al. (2024) studied this question.

synapsesocial.com/papers/6a7002465d37378ac1dd0fe4 https://doi.org/10.1155/adpp/9076988

View Full Paper

Ask AI

Bookmark

Implication

In vitro study demonstrates that octanoic and decanoic acids reduce tunicamycin-induced cellular stress in rat aortic smooth muscle cells, indicating a potential protective therapy for vascular...

Octanoic Acid and Decanoic Acid Inhibit Tunicamycin‐Induced ER Stress in Rat Aortic Smooth Muscle Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Octanoic Acid and Decanoic Acid Inhibit Tunicamycin‐Induced ER Stress in Rat Aortic Smooth Muscle Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider