What question did this study set out to answer?

This study aims to explore the connection between the oxidation state of the aconitase iron-sulfur cluster and its enzymatic activity and substrate binding.

December 1, 1981Biochemistry

Relationship of the oxidation state of the iron sulfur cluster of aconitase to activity and substrate binding

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This study aims to explore the connection between the oxidation state of the aconitase iron-sulfur cluster and its enzymatic activity and substrate binding.
Aconitase was isolated and assessed for activity under varying conditions with and without iron and reducing agents.
Coulometric activation was measured to observe the role of the iron-sulfur cluster in catalytic activity.
Substrate binding capacity was analyzed for both active and inactive conformations.
Full activation of aconitase occurs without external iron when 3Fe and 3S are present, indicating the role of reduction.
Catalytic activity shows a lag following reduction of the iron-sulfur cluster.
Active aconitase specifically binds a transition-state analogue but is unable to do so in its inactive conformation.

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Implication

Randomized trial investigates aconitase activity in relation to iron-sulfur cluster, suggesting conformational change implications.

Cite This Study

Ramsay et al. (1981) studied this question.

synapsesocial.com/papers/6a700b22e71d69abee085763 https://doi.org/10.1021/bi00529a023

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Relationship of the oxidation state of the iron sulfur cluster of aconitase to activity and substrate binding

Key Points

Discussion

Implication

Cite This Study

Relationship of the oxidation state of the iron sulfur cluster of aconitase to activity and substrate binding

Key Points

Discussion

Implication

Cite This Study

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