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This research aims to explore how iron influences the activation and inactivation of aconitase.

The role of iron in the activation-inactivation of aconitase.

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Randomized trial demonstrates aconitase activation in various conditions, suggesting alternative pathways for iron involvement.

This research aims to explore how iron influences the activation and inactivation of aconitase.
Aconitase was prepared from beef heart mitochondria in both inactive [3Fe-4S] and active [4Fe-4S] forms.
Activation was observed under anaerobic conditions using reducing agents without added iron, and Fe2+ requirements were assessed.
EPR (Electron Paramagnetic Resonance) was used to quantify cluster changes and iron incorporation.
Aconitase activation without added iron reached 70-80% of maximal activity, indicating cluster rebuilding from [3Fe-4S] to [4Fe-4S].
Incorporation of 59Fe showed rapid binding of iron atoms per cluster, with longer incubations revealing iron presence in multiple cluster sites.
Oxidation was found to initiate inactivation, leading to loss of iron and formation of [3Fe-4S] clusters.

Kennedy et al. (1983) studied this question.

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