What question did this study set out to answer?

To precisely establish the molecular weight and elemental iron and labile sulfide stoichiometry in beef heart aconitase to evaluate the proposed [3Fe-4S] cluster model.

March 1, 1984Journal of Biological ChemistryOpen Access

Molecular weight of beef heart aconitase and stoichiometry of the components of its iron-sulfur cluster.

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Authors

LRLars RydénPreventive Cardiology LÖLars‐Göran ÖfverstedtOkinawa Institute of Science and Technology Graduate University HBHelmut BeinertThe University of Queensland

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Implication

Biochemical analysis demonstrates a 3-to-4 iron-sulfur stoichiometry in beef heart aconitase, confirming the presence of a single [3Fe-4S] cluster in the inactive enzyme.

Key Points

To precisely establish the molecular weight and elemental iron and labile sulfide stoichiometry in beef heart aconitase to evaluate the proposed [3Fe-4S] cluster model.
Determined molecular weight using low-speed sedimentation equilibrium, gel chromatography in 6 M guanidine HCl, and SDS gel electrophoresis.
Quantified protein concentration via amino acid analysis and measured iron and labile sulfide content across 20 enzyme preparations of at least 95% purity.
Sedimentation equilibrium established an enzyme molecular weight of 80,900 ± 2,200, matching gel chromatography (78,400 Mr; single peptide of 710 residues) and SDS-PAGE (83,000 Mr).
Elemental quantification across 20 preparations yielded 2.9 ± 0.2 Fe/mol and 3.9 ± 0.2 S2-/mol, validating the [3Fe-4S] cluster model for inactive aconitase.

Cite This Study

Rydén et al. (1984) studied this question.

synapsesocial.com/papers/6a700b906ceb2bbd16dfc734 https://doi.org/10.1016/s0021-9258(17)43272-8

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Implication

Biochemical analysis demonstrates a 3-to-4 iron-sulfur stoichiometry in beef heart aconitase, confirming the presence of a single [3Fe-4S] cluster in the inactive enzyme.

Molecular weight of beef heart aconitase and stoichiometry of the components of its iron-sulfur cluster.

Discussion

Member takes

Implication

Key Points

Cite This Study

Molecular weight of beef heart aconitase and stoichiometry of the components of its iron-sulfur cluster.

Discussion

Member takes

Implication

Key Points

Cite This Study

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