Why the study?
Does an enzyme immunoassay using monoclonal antibodies against Norwalk virus accurately detect the virus in stool specimens?
Does an enzyme immunoassay using monoclonal antibodies against Norwalk virus accurately detect the virus in stool specimens?
Monoclonal antibodies against Norwalk virus can be used in an enzyme immunoassay for sensitive and specific detection of the virus in stool samples.
This is the authors' abstract. We don't add key points for this paper.
Monoclonal antibodies against the prototype 8FIIa strain of Norwalk virus were prepared and applied to an enzyme immunoassay (EIA) for detecting Norwalk virus in stool specimens. The monoclonal antibodies immunoprecipitated a 58-kDa protein which had been produced by in vitro transcription-translation of Norwalk virus cloned cDNA, and they reacted by EIA with recombinant Norwalk virus capsid protein at a sensitivity level of 1 ng/ml. The EIA detected virus in all tested samples from 15 different Norwalk virus-infected volunteers. No cross-reactions were seen in stools containing other caliciviruses or in stools containing rotaviruses, astroviruses, or enteric adenoviruses.
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May enable EIA detection of Norwalk virus in stool; leaves open clinical adoption pending prospective validation.
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Herrmann et al. (1995) studied this question.
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