Population
BHK-21 cells infected with a vaccinia virus expressing T7 RNA polymerase and transfected with astrovirus-VP4…
Comparison
Different regions of the human astrovirus… vs Cell-free translation system and constructs with…
Design
Preclinical
Authors
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Cell-free systems may underestimate astrovirus frameshifting; leaves open pseudoknot necessity in intact replication contexts.
Astrovirus ribosomal frameshifting efficiency is significantly higher in an intact-cell system compared to a cell-free system and requires the shifty heptamer but not the downstream pseudoknot region.
Lewis et al. (1996) studied this question.
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