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September 1, 1994Journal of VirologyOpen Access

The human astrovirus RNA-dependent RNA polymerase coding region is expressed by ribosomal frameshifting

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Population

cDNA fragment containing the 1a-1b overlap region of human astrovirus serotype 1 cloned within a reporter…

Design

Preclinical

Authors

BMBeate MarczinkeABAlison J BloysTBT. D. K. BrownUniversity of Cambridge

Discussion

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Overview

Informs antiviral target design for astrovirus; leaves open validation in human models before any clinical consideration.

Structured PICO

P
Population
cDNA fragment containing the 1a-1b overlap region of human astrovirus serotype 1 (HAst-1) cloned within a reporter gene and placed under the control of the bacteriophage SP6 promoter in a recombinant plasmid, translated in the rabbit reticulocyte lysate cell-free system
I
Intervention
Mutation analysis and direct structure probing with single- and double-stranded RNA-specific reagents
O
Outcome
Expression of ORF 1b mediated through -1 ribosomal frameshiftingsurrogate

The study demonstrates that the human astrovirus RNA-dependent RNA polymerase coding region is expressed via a -1 ribosomal frameshift mechanism involving a slippery sequence and a stem-loop structure, without an RNA pseudoknot.

Cite This Study

Marczinke et al. (1994) studied this question.

synapsesocial.com/papers/6a7e605a4928f3552d3c3c19https://doi.org/10.1128/jvi.68.9.5588-5595.1994
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1RNA sequence of astrovirus: distinctive genomic organization and a putative retrovirus-like ribosomal frameshifting signal that directs the viral replicase synthesis.1993 · 287 citations
  2. 2Analysis of astrovirus serotype 1 RNA, identification of the viral RNA-dependent RNA polymerase motif, and expression of a viral structural protein1994 · 146 citations
  3. 3An RNA pseudoknot and an optimal heptameric shift site are required for highly efficient ribosomal frameshifting on a retroviral messenger RNA.1992 · 174 citations
  4. 4Ribosomal Pausing during Translation of an RNA Pseudoknot1993 · 104 citations
  5. 5The human immunodeficiency virus type 1 packaging signal and major splice donor region have a conserved stable secondary structure1992 · 204 citations