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calculating the extra rate in this unit at a series of instants during the course of the manometric experi- ment. These extra rates are then extrapolated to zero time so as to eliminate changes in the activity of the enzyme during the actual experiment owing to (i) progressive alterations in pH, substrate and buffer concentrations as C02 is taken up or evolved, (ii) destruction brought about bytheviolent shaking.
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Roughton et al. (1946) studied this question.
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