What question did this study set out to answer?

To establish and validate a quantitative method for measuring in vivo glucose utilization in skeletal muscle and white adipose tissue of anaesthetized rats.

May 15, 1985Biochemical JournalOpen Access

A method to quantify glucose utilization in vivo in skeletal muscle and white adipose tissue of the anaesthetized rat

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Authors

PFPascal FerréInserm ALArmelle LeturqueInserm ABA. F. BurnolCentre National de la Recherche Scientifique

Discussion

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Implication

In vivo experimental study demonstrates accurate measurement of tissue-specific glucose utilization in anaesthetized rats, indicating reliable evaluation across varied metabolic states.

Key Points

To establish and validate a quantitative method for measuring in vivo glucose utilization in skeletal muscle and white adipose tissue of anaesthetized rats.
Administered an intravenous 2-deoxy[3H]glucose tracer into anaesthetized rats and monitored tracer clearance kinetics in arterial blood.
Excised skeletal muscles (soleus, extensor digitorum longus, epitrochlearis), periovarian white adipose tissue, and brain after 30 to 80 minutes to measure accumulated 2-deoxy[3H]glucose 6-phosphate using in vitro correction factors for transport and phosphorylation.
Assessed glucose utilization across three distinct physiological states: basal post-absorptive state, euglycaemic-hyperinsulinaemic clamp (8 munits insulin/ml), and 48-hour starvation.
Tracer accumulation in tissues mapped quantitatively to local glucose utilization when calculated against the arterial blood clearance integral and analogue correction factors.
Tissue glucose utilization measurements qualitatively and quantitatively aligned with expected physiological shifts between basal, insulin-stimulated, and starvation states.

Cite This Study

Ferré et al. (1985) studied this question.

synapsesocial.com/papers/6a70175a26a7f98052dc997b https://doi.org/10.1042/bj2280103

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May 15, 1985Biochemical JournalOpen Access

A method to quantify glucose utilization in vivo in skeletal muscle and white adipose tissue of the anaesthetized rat

View Full Paper

Ask AI

Bookmark

Authors

PFPascal FerréInserm ALArmelle LeturqueInserm ABA. F. BurnolCentre National de la Recherche Scientifique

Discussion

Member takes

Implication

In vivo experimental study demonstrates accurate measurement of tissue-specific glucose utilization in anaesthetized rats, indicating reliable evaluation across varied metabolic states.

Key Points

To establish and validate a quantitative method for measuring in vivo glucose utilization in skeletal muscle and white adipose tissue of anaesthetized rats.
Administered an intravenous 2-deoxy[3H]glucose tracer into anaesthetized rats and monitored tracer clearance kinetics in arterial blood.
Excised skeletal muscles (soleus, extensor digitorum longus, epitrochlearis), periovarian white adipose tissue, and brain after 30 to 80 minutes to measure accumulated 2-deoxy[3H]glucose 6-phosphate using in vitro correction factors for transport and phosphorylation.
Assessed glucose utilization across three distinct physiological states: basal post-absorptive state, euglycaemic-hyperinsulinaemic clamp (8 munits insulin/ml), and 48-hour starvation.
Tracer accumulation in tissues mapped quantitatively to local glucose utilization when calculated against the arterial blood clearance integral and analogue correction factors.
Tissue glucose utilization measurements qualitatively and quantitatively aligned with expected physiological shifts between basal, insulin-stimulated, and starvation states.

Cite This Study

Ferré et al. (1985) studied this question.

synapsesocial.com/papers/6a70175a26a7f98052dc997b https://doi.org/10.1042/bj2280103

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