Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
May 1, 1989Journal of Biological ChemistryOpen Access

Differential modulation of actin-severing activity of gelsolin by multiple isoforms of cultured rat cell tropomyosin

View Full Paper
Ask AI
Bookmark
Share

Population

Actin filaments from cultured rat cells

Comparison

Tropomyosin isoforms and nonmuscle caldesmon vs Control actin filaments or actin filaments…

Design

Preclinical

Authors

RIReina IshikawaWaseda UniversitySYShigeko YamashiroRutgers, The State University of New JerseyFMFumio MatsumuraRutgers, The State University of New Jersey

Discussion

Loading...

Member takes

Implication

May stabilize actin in vascular cells; leaves open relevance to human cardiovascular contractility.

Structured PICO

P
Population
Actin filaments from cultured rat cells
I
Intervention
Tropomyosin (TM) isoforms (high Mr and low Mr) and nonmuscle caldesmon
C
Comparator
Control actin filaments or actin filaments exposed to gelsolin alone
O
Outcome
Actin-severing activity of gelsolin (measured by average length of actin filaments via electron microscopy and flow birefringence)surrogate

High Mr tropomyosin isoforms, especially when combined with nonmuscle caldesmon, protect actin filaments from fragmentation by gelsolin, suggesting they share the same binding site on actin.

Cite This Study

Ishikawa et al. (1989) studied this question.

synapsesocial.com/papers/6a702ce7b27f158178273ae4https://doi.org/10.1016/s0021-9258(18)83261-6
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Modulation of actin-bundling activity of 55-kDa protein by multiple isoforms of tropomyosin.1986 · 32 citations
  2. 2Differential expression of tropomyosin forms in the microfilaments isolated from normal and transformed rat cultured cells.1983 · 216 citations
  3. 3Tropomyosin isoforms in chicken embryo fibroblasts: purification, characterization, and changes in Rous sarcoma virus-transformed cells.1985 · 124 citations
  4. 4Purification and characterization of an F-actin-bundling 55-kilodalton protein from HeLa cells.1985 · 123 citations
  5. 5Tropomyosin isoform switching in tumorigenic human fibroblasts.1986 · 84 citations