What question did this study set out to answer?

Determine whether human osteosarcoma cells transcribe the c-sis proto-oncogene and synthesize proteins related to platelet-derived growth factor (PDGF).

November 23, 1984Science

Detection of c- sis Transcripts and Synthesis of PDGF-Like Proteins by Human Osteosarcoma Cells

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Authors

DGDana T. GravesUniversity of Pennsylvania AOAlbert J. OwenBoston University RBRichard K. BarthCalifornia Institute of Technology

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Implication

Laboratory study demonstrates c-sis transcription and PDGF-like protein secretion in human osteosarcoma cells, suggesting an autocrine mechanism in mesenchymal tumor development.

Key Points

Determine whether human osteosarcoma cells transcribe the c-sis proto-oncogene and synthesize proteins related to platelet-derived growth factor (PDGF).
Analyzed the human osteosarcoma cell line U-2 OS for the presence of c-sis RNA transcripts.
Performed immunoprecipitation on U-2 OS cell extracts and conditioned media using specific antiserum raised against PDGF.
U-2 OS osteosarcoma cells expressed multiple distinct c-sis transcripts.
PDGF antiserum specifically immunoprecipitated intracellular polypeptides ranging in molecular weight from 18,000 to 165,000 daltons from cell extracts.
Cells secreted a 29,000-dalton protein exhibiting structural and serological characteristics of PDGF.

Cite This Study

Graves et al. (1984) studied this question.

synapsesocial.com/papers/6a7030508031ec7bb1dc2cec https://doi.org/10.1126/science.6209798

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Implication

Laboratory study demonstrates c-sis transcription and PDGF-like protein secretion in human osteosarcoma cells, suggesting an autocrine mechanism in mesenchymal tumor development.

Detection of c- sis Transcripts and Synthesis of PDGF-Like Proteins by Human Osteosarcoma Cells

Discussion

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Implication

Key Points

Cite This Study

Detection of c- sis Transcripts and Synthesis of PDGF-Like Proteins by Human Osteosarcoma Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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