What question did this study set out to answer?

To determine whether the absence of detectable PDGF receptors in simian sarcoma virus-transformed cells is caused by continuous autocrine ligand binding and receptor downregulation.

December 1, 1984Proceedings of the National Academy of SciencesOpen Access

Blockade of autocrine stimulation in simian sarcoma virus-transformed cells reverses down-regulation of platelet-derived growth factor receptors.

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To determine whether the absence of detectable PDGF receptors in simian sarcoma virus-transformed cells is caused by continuous autocrine ligand binding and receptor downregulation.
Assayed conditioned media from simian sarcoma virus (SSV)-transformed cells using 125I-labeled PDGF competition binding, DNA synthesis assays, and tyrosine phosphorylation assays in normal cells.
Assessed surface PDGF receptor levels and receptor phosphorylation in normal and SSV-transformed cells using anti-phosphotyrosine antibodies and radioligand binding.
Treated SSV-transformed cells with suramin, an inhibitor of PDGF-receptor binding, to track the kinetics of receptor recovery over time.
Conditioned media and co-culture with SSV-transformed cells stimulated DNA synthesis, triggered tyrosine phosphorylation, and downregulated PDGF receptors in normal cells.
Untreated SSV-transformed cells displayed no detectable surface PDGF receptors via anti-phosphotyrosine antibodies or 125I-PDGF binding.
Suramin treatment blocked autocrine PDGF binding and restored functional, phosphorylatable surface PDGF receptors to control levels within 8 hours.

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Implication

In vitro experiment reveals suramin restores PDGF receptors in virus-transformed cells, indicating autocrine signaling drives receptor downregulation.

Cite This Study

Garrett et al. (1984) studied this question.

synapsesocial.com/papers/6a70310675498292b70992e5 https://doi.org/10.1073/pnas.81.23.7466

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Implication

In vitro experiment reveals suramin restores PDGF receptors in virus-transformed cells, indicating autocrine signaling drives receptor downregulation.

Blockade of autocrine stimulation in simian sarcoma virus-transformed cells reverses down-regulation of platelet-derived growth factor receptors.

Key Points

Discussion

Implication

Cite This Study

Blockade of autocrine stimulation in simian sarcoma virus-transformed cells reverses down-regulation of platelet-derived growth factor receptors.

Key Points

Discussion

Implication

Cite This Study

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