Multi-Stimuli-Responsive Biocompatible Magnetic Nanocarrier as Drug delivery System to MC7 Breast Cancer Cells

Abstract Efforts are ongoing to develop effective controlled-release therapeutic systems through innovative functional targeted drug delivery systems (TDDS). Functional polymeric nanocarriers are crucial in delivering therapeutic drugs efficiently into tumors. This study designed a redox/pH-responsive functional drug delivery platform with ligands for targeting MC7 breast cancer cells using a core-shell biocompatible magnetic nanocarrier. The process involved synthesizing oleic acid-coated Fe3O4@SiO2 nanoparticles, modifying pluronic F127 (PF 127) with maleic anhydride and aminating it to form PF127-NH2, linking L-cysteine to PF127-NH2, and condensing carboxymethyl-β-cyclodextrin (CM-β-CD) to PF127-NH2/L-cysteine, which was targeted by folic acid (FA). The resulting PF127-NH2/L-cysteine-CM-β-CD-FA formed LCMNPs-SS in the presence of oleic acid-coated Fe3O4@SiO2. Doxorubicin (DOX) was used as a model drug and loaded into the LCMNPs-SS nanocarrier. The LCMNPs-SS nanocarrier exhibited excellent properties for controlled release, with release rate and level controllable by adding an external magnet and adjusting the DTT concentration. Additionally, the LCMNPs-SS nanocarrier could disassemble under the effects of oxidant or pH, allowing for fine-tuning of drug release in response to temperature and pH or multiple stimuli.